Molecular Marker Modules Vol 1

Using Molecular Marker Technology in Studies on Plant Genetic Diversity:
Learning Module
Volume 1
(caution: some files are large, and may not be as interactive as the CD)

Title page [PDF, 115 kb]
Preface [PDF, 9 kb]
Foreword [PDF, 11 kb]
About this module [PDF, 13 kb]
Objectives [PDF, 7 kb]

I. Introduction [PDF, 486 kb]

II. Protein-based technologies

* Protein basics [PDF, 137 kb]
* Isozymes [PDF, 295 kb]

III. DNA-based technologies

* DNA basics [PDF, 394 kb]
* Restriction fragment length polymorphisms (RFLPs) [PDF, 503 kb]
* PCR basics [PDF, 326 kb]
* PCR with arbitrary primers [PDF, 206 kb]
* Amplified fragment length polymorphisms (AFLPs) [PDF, 279 kb]
* Sequence-tagged sites (Microsatellites etc) [PDF, 308 kb]
* Latest strategies [PDF, 324 kb]

IV. Complementary technologies [PDF, 136 kb]

*Denaturing gradient gel electrophoresis (DGGE)
* Thermal gradient gel electrophoresis (TGGE)
* Single-stranded conformational polymorphism (SSCP)
* Heteroduplex analysis
* Denaturing high-performance liquid chromatography (DHPLC)

V. Final considerations [PDF, 152 kb]

* Considerations when choosing a technique
* Practical applications

VI.Glossary [PDF, 33 kb]
VII. Feedback form [PDF, 14 kb]
VIII. Acknowledgements [PDF, 11 kb]
IX. Copyright [PDF, 50 kb]

Download whole module [zipped file PDF, 2.7 Mb]
Read Table of Contents and download PDF files of Volume 2