A CGIAR Generation Challenge Programme project.....Cultivating Genetic Diversity for the Resource Poor
 

 
Home
Sorghum & Millet
Helpdesk
About IRC
Contact Us
Resources for Scientists
Databases
Literature & News
Protocols
Molecular Marker Modules
Tutorials 
Funding Training Visits
Funding for Research
African Molecular Marker Net
ABNETA
Dictionaries, Glossaries
Bioinformatics Resources
Frequently Asked Questions
GCP IP Helpdesk
Teaching Resources
Forum
Find people
Find Lab Products
Favorite Websites
 
The Laboratory Manual from the 2007 Marker Assisted Selection Training Course in Ghana. Download as a Word file. This includes DNA extractions, SSR PCR, AFLPs, and gels.

newTomato Transformation protocol from Joyce van Eck (Boyce Thompson Inst, Cornell University). View or download Word file.


Protocol Online , protocols and lab safety info, how to keep a lab notebook, and more


Fulton TM, Chunwongse J, and Tanksley SD. (1995) Microprep Protocol for Extraction of DNA from Tomato and other Herbaceous Plants. Plant Molecular Biology Reporter 13 (3): 207-209. See the protocol here or download a pdf of the article.

Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. (Phytochemical Bulletin 19: 11-15). Download pdf (from Doyle lab)
DNA Extraction Protocol using CTAB and GenoGrinder, from the IGD lab
DNA Extraction Protocol for lyophilized tissue, from the IGD lab
Principles of PCR (from Martha Hamblin, IGD lab)

Lab Manual from GCP-Pretoria, South Africa training course, including Safety Practices, DNA extraction, PCR, SSRs, etc. (Thanks to Andrew Kiggundu[Uganda], Chris Cullis[US] and Karl Kunert[FABI, SA]).

Basic Bacterial Mini-prep protocol, from the IGD lab

A list of protocols compiled by G. Lazo at USDA: http://wheat.pw.usda.gov/~lazo/methods/

Also see the DNA Extraction Troubleshooting page  
               
 
Have a Question or a Problem?
Email the helpdesk